Document Type : Original article

Authors

• Samaneh Saedi ¹
• kiarash ghazvini ²
• Masoud Youssefi ³
• Hadi Safdar ¹
• saman soleimanpour ¹
• Parviz Afrough ¹
• Amir Azimian ⁴
• hamid solgi ⁵
• Masoud Keikha ⁶

¹ Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

² Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

³ Department of Microbiology and Virology, Mashhad University of Medical Sciences, Mashhad, Iran.

⁴ Microbiology and Virology Research Centre, department of Bojnord University of Medical Sciences, Bojnord, Iran

⁵ Departmant of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran. Microbiology Research Center (MRC) Pasteur Institute of Iran.

⁶ Department of Microbiology and Virology, Faculty of Medicine, Iranshahr University of Medical Sciences, Iranshahr, Iran

Abstract

Abstract
Background: Mycobacterium tuberculosis is still one of the most dangerous human pathogens. Identification of the relationships between different clinical strains has remained a high priority for epidemiology research.
Methods: In this study, we used MLSA (Multilocus sequence analysis) to generate a highly robust phylogeny of M. tuberculosis. MLSA, based on single nucleotide polymorphism (SNP) was performed on five genes fragments from the Rpsl (302 bp), MprA (559 bp), LipR (322 bp), KatG (488 bp) and Fgd1 (266 bp), in order to identify polymorphic nucleotide sites, and the discriminatory power of each locus for all genes was measured with Hunter‐Gaston Index (HGI).
Results: In this study, a sequence type (ST) number was assigned to each unique allelic profile, and 9 sequence types were identified from 20 strains, these imply that there is a high diversity of strains in this area.
Conclusion: Our results showed that the presence of high genetic diversity among clinical isolates of M. tuberculosis in Northeast of Iran. There is no evidence for recent transmission.
Keywords: Mycobacterium tuberculosis, Multi-locus sequence analysis; Molecular epidemiology; Tuberculosis; KatG; Rpsl
1. Introduction
Mycobacterium tuberculosis (M. tuberculosis), the causative agents of tuberculosis (TB), is one of the most successful human pathogens, infecting nearly one-third of the people all around the world, causing over 9 million new cases and 1.7 million deaths each year [1-2]. Identification of the relationships between different clinical strains of M. tuberculosis has great significance to the public health [3].

Keywords

• Mycobacterium tuberculosis, Multi-locus sequence analysis
• Molecular epidemiology
• Tuberculosis
• KatG
• Rpsl